A new research protocol developed at Stanford University in California improves on their previous technological breakthrough and lets neuroscientists visualize a brain across multiple scales, says program manager Dr. Justin Sanchez.
Stanford scientists earlier developed a method called Clear, Lipid-exchanged, Acrylamide-hybridized Rigid, Imaging/immunostaining compatible, Tissue hydrogel (CLARITY) to study brain tissue. The method uses a chemical to transform intact biological tissues into a hydrogel hybrid, which makes the brain tissues transparent.
“Brains are not clear to begin with, therefore if you’re trying to use, let’s say, a microscope to study the volume of tissue of the brain, the light can’t transverse through all of the structure,” Sanchez explains. “However, if you do ‘clarify’ it using this very novel technique, then you are able to get through a volume of tissue and study all of the circuitry.”
Under the CLARITY protocol, Sanchez says, it could take upwards of 80 years to conduct the imaging process for a complete human brain. But the new protocol accelerates the process so that the same technique now takes 220 days.